Using negative oxygen ion data from three monitoring stations (Tianyuan Peony Base, Qianchi Park, and Heizhugou Scenic Area) in Ebian County from April 2021 to March 2024, combined with concurrent meteorological observations from national meteorological stations, the variation characteristics of negative oxygen ion concentration and its meteorological influencing factors are analyzed. The results show that the negative oxygen ions in Ebian County show a single-peak diurnal variation characteristic of high during the day and low at night, the M-shaped monthly variation trend of one main peak and one secondary peak, and the seasonal differences that negative oxygen ion concentration in spring and summer is higher than that in autumn and winter. Taking Qianchi Park as an example, the negative oxygen ion concentration at the site is positively correlated with temperature, precipitation, and wind speed, but negatively correlated with humidity and sunshine.