Abstract: Based on satellite remote sensing data and meteorological data, remote sensing ecological environment index (RSEI) and biodiversity maintenance function index (BMI) were constructed to evaluate the ecological environment condition and the biodiversity maintenance function of Daxue Mountains ecological red line area from 2000 to 2019, and the climate change characteristics in the red line area were also analyzed. The results showed that: (1) From 2000 to 2019, the overall ecological environment showed a good trend, and the area proportion of RSEI level of general and above reached to 90%. There were obvious differences of ecological environment condition in the red line area. The ecological environment in the north and central areas was good, and the ecological environment in the southeast was poor. (2) From 2000 to 2019, the biodiversity function in the red line area continued to improve, and the area proportion of BMI level of general and above reached to 84.912%. The distribution of BMI of generally or above level was mainly located in the northern, central and western parts in the red line area, and the distribution of poor and worse level was mainly located in the southeast of the red line area. (3) Since 1990, there has been an aboriginal warming, sufficient precipitation, a slight increase in sunshine and a slight decrease in wind speed in the red line area of the Daxue Mountains. Warm and humid climate conditions are conducive to the ecological restoration of the red line area, and the ecological environment and ecological functions tend to improve.